RESUMEN
Ozone therapy's efficacy might stem from the regulated and mild oxidative stress resulting from ozone's interactions with various biological elements. The present work aimed to characterize the hepatic mitochondrial response to ozone treatment and its relationship with the antioxidant system response. Two groups of mice were used: one control group and another injected intraperitoneally with an O3/O2 mixture (80â¯ml/kg) for 5 days. Mitochondrial respiration supported by different substrates was significantly inhibited, as well as complexes I and II/III, but not complex IV. The analysis of the electron transport chain complex activity showed significant inhibitions in complexes I and II/III but not in complex IV. These inhibitions can prevent mitochondrial reactive oxygen species (ROS) production. Additionally, there was a decline in glutathione content, unaccompanied by a rise in its oxidized form. The ozone-treated groups showed a significant increase in the activity of superoxide dismutase and glutathione peroxidase, while catalase and glutathione reductase experienced no significant alterations. Adenine nucleotides increased in the ozone group, but only the increase in adenosine diphosphate is significant, so the cell's energy charge is unaffected. This study shows that mitochondria may play a crucial role in ozone treatment. However, it also highlights the need for further studies to understand the molecular mechanism.
RESUMEN
Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant Staphylococcus aureus (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Therefore, new alternatives are urgently needed. Thus, this study aimed to investigate the capacity of topical ozonated oil application on the treatment of early-stage skin infected with MRSA in an animal model. Ozonated oil was prepared from a mixture of oils subjected to a gas stream of O2/O3 mixture. Sixteen Wistar rats were inoculated by an intradermic injection of MRSA suspension, producing an abscess lesion. After 3 days, the skin epidermis was removed to open the wound. Group 1 received an application of oil mixture without ozone treatment and Group 2 received an application of ozonated oil. After the treatment period, skin was collected, colony-forming units (CFU) of bacteria were quantified and the histological analysis of the skin was carried out. Skin samples from the control 1 and 2 had a bacterial load was of 1.1 × 105 and 5.7 × 103 CFU/mL, respectively. Group 2 showed better wound healing from mild to moderate epidermal regeneration. Topical application of ozonated vegetable oil in MRSA-infected skin in rats showed a small reduction of the bacterial load and better wound healing.
RESUMEN
Ozone has a high wound healing capacity and antibacterial properties and can be used as a complementary treatment in infections. Methicillin-resistant S. aureus (MRSA) is the most common pathogen found in infected diabetic foot ulcers. Most of MRSA are resistant to several classes of antibiotics and, therefore, there is a need for new, effective, and well-tolerated agents. Thus, we aimed evaluate the antimicrobial and antibiofilm potentials of ozonated vegetable oils against MRSA strains isolated from diabetic foot ulcers. Six ozonated oils were produced with concentrations of ozone ranging from 0.53 to 17 mg of ozone/g of oil. The peroxide values were determined for each oil. Ozonated oils content on fatty acid was determined by gas chromatography equipped with a flame ionization detector. The antimicrobial susceptibility testing was performed by the Kirby-Bauer disk diffusion method and the effect of ozonated oils on biofilm formation ability and on established biofilms was investigated. In general, the content in identified unsaturated fatty acid in oils decreased with the increase of ozonation time and, consequently, the peroxide value increased. Most bacterial strains were inhibited by ozonated oil at a concentration of 4.24 mg/g. Ozonated oils had moderate to high ability to remove adhered cells and showed a high capacity to eradicate 24 h old biofilms. Our results show promising use of ozonated oils on the treatment of infections, in particular those caused by multidrug-resistant MRSA strains.
Asunto(s)
Biopelículas/efectos de los fármacos , Pie Diabético/microbiología , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Staphylococcus aureus Resistente a Meticilina/fisiología , Aceites/química , Ozono/química , Ozono/farmacología , Antibacterianos/química , Antibacterianos/farmacología , Adhesión Celular/efectos de los fármacos , Pruebas de Sensibilidad MicrobianaRESUMEN
El presente estudio, transversal y observacional, estuvo referido a las caracteristicas de la postura corporal en niños respiradores bucales. El mismo se realizo en 24 pacientes del Servicio de ORL en el Hospital Dr. Reynaldo Barrionuevo en Capitan Bermudez, durante agosto-febrero de 2000/01. Las edades de los pacientes estuvieron comprendidas entre 5 y 15 años , la totalidad de ellos preentaban respiracion bucal y fueron diagnosticados por el otorrinolaringologo. Sobre las fotografias de cada paciente se realizaron una serie de trazados lineales y mediciones para poder investigart los diferentes aspectos de la postura corporal. En la alineacion corporal, la alineacion de la cabeza con respecto al tronco, los hombros, la pelvis, las rodillas y los pies, se encontro que la mayoria de los niños presentaron no alineacion. En cuanto al angulo de la talla prevalecio la asimetria y las curvaturas vertebrales se observaron alteradas en la gran mayoria de los pacientes. Respecto del torax se presento normal en casi todos los casos y el abdomen se observo plano tambien en la mayoria de los niños (mayores de 11 años). Relacionando las variables entre si se encontraron asociaciones estadisticamente significativas en las siguientes: . Alineacion corporal - Angulo de la talla: la ausencia o asimetria del angulo fue mas frecuente entre los no alineados. . Hombros - escapulas: la no alineacion en hombros fue mas frecuente entre los no alineados para escapulas. Esta investigacion pone en evidencia que generalmente los respiradores bucales presentan una postura corporal desorganizada, ya que la mayoria de los aspectos que componen la misma se presentaron en situaciones o valores no adecuados
Asunto(s)
Humanos , Adolescente , Niño , Estudios Transversales , Epidemiología Descriptiva , Boca , Postura , Respiración , Signos y Síntomas , SomatotiposRESUMEN
El presente estudio, transversal y observacional, estuvo referido a las caracteristicas de la postura corporal en niños respiradores bucales. El mismo se realizo en 24 pacientes del Servicio de ORL en el Hospital Dr. Reynaldo Barrionuevo en Capitan Bermudez, durante agosto-febrero de 2000/01. Las edades de los pacientes estuvieron comprendidas entre 5 y 15 años , la totalidad de ellos preentaban respiracion bucal y fueron diagnosticados por el otorrinolaringologo. Sobre las fotografias de cada paciente se realizaron una serie de trazados lineales y mediciones para poder investigart los diferentes aspectos de la postura corporal. En la alineacion corporal, la alineacion de la cabeza con respecto al tronco, los hombros, la pelvis, las rodillas y los pies, se encontro que la mayoria de los niños presentaron no alineacion. En cuanto al angulo de la talla prevalecio la asimetria y las curvaturas vertebrales se observaron alteradas en la gran mayoria de los pacientes. Respecto del torax se presento normal en casi todos los casos y el abdomen se observo plano tambien en la mayoria de los niños (mayores de 11 años). Relacionando las variables entre si se encontraron asociaciones estadisticamente significativas en las siguientes: . Alineacion corporal - Angulo de la talla: la ausencia o asimetria del angulo fue mas frecuente entre los no alineados. . Hombros - escapulas: la no alineacion en hombros fue mas frecuente entre los no alineados para escapulas. Esta investigacion pone en evidencia que generalmente los respiradores bucales presentan una postura corporal desorganizada, ya que la mayoria de los aspectos que componen la misma se presentaron en situaciones o valores no adecuados(AU)
